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- Title
Recombination adenovirus-mediated human lactoferrin cDNA inhibits the growth of human MCF-7 breast cancer cells.
- Authors
Wang, Jianjie; Li, Qingwang; Ou, Yetao; Li, Kun; Han, Zengsheng; Wang, Peijun; Zhou, Shaobo
- Abstract
Objectives Human lactoferrin, an 80 kDa iron-binding glycoprotein, has antitumour effects. We have explored the potential therapeutic role of re-expressing human lactoferrin gene product in human breast cancer. Methods A recombinant adenovirus expressing the human lactoferrin cDNA (ad-hLTF) was constructed and used to infect breast cancer cells. Key findings Seventy-two hours after infection, ad-hLTF had considerable cytotoxicity on MCF-7 cells. A time-course study showed that ad-hLTF infection of MCF-7 cells at 100 plaque-forming units per cell increased the number of cells in G0/G1 phase and appeared markedly at Sub-G1 apoptotic peak. The presence of apoptotic cells was confirmed using Annexin V-fluoresecein isothiocyanate apoptosis detection by flow cytometry. Ad-hLTF also resulted in a decrease of Bcl-2 protein and an increase in Bax protein. Conclusions Ad-hLTF plays an important role in the induction of cell cycle arrest and apoptosis in MCF-7 cells. The results demonstrated that ad-hLTF could have potential benefits in the treatment of breast cancer.
- Subjects
APOPTOSIS; BREAST cancer; LACTOFERRIN; ADENOVIRUSES; CANCER cells
- Publication
Journal of Pharmacy & Pharmacology, 2012, Vol 64, Issue 3, p457
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/j.2042-7158.2011.01410.x