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- Title
Endolithic Microbial Habitats Hosted in Carbonate Nodules Currently Forming within Sediment at a High Methane Flux Site in the Sea of Japan.
- Authors
Yanagawa, Katsunori; Shiraishi, Fumito; Tanigawa, Yusuke; Maeda, Toshinari; Mustapha, Nurul Asyifah; Owari, Satoko; Tomaru, Hitoshi; Matsumoto, Ryo; Kano, Akihiro
- Abstract
Concretionary carbonates in deep-sea methane seep fields are formed as a result of microbial methane degradation, called anaerobic oxidation of methane (AOM). Recently, active microorganisms, including anaerobic methanotrophic archaea, were discovered from methane seep-associated carbonate outcroppings on the seafloor. However sedimentary buried carbonate nodules are a hitherto unknown microbial habitat. In this study, we investigated the microbial community structures in two carbonate nodules collected from a high methane flux site in a gas hydrate field off the Oki islands in the Sea of Japan. The nodules were formed around sulfate-methane interfaces (SMI) corresponding to 0.7 and 2.2 m below the seafloor. Based on a geochemical analysis, light carbon isotopic values ranging from −54.91‰ to −37.32‰ were found from the nodules collected at the shallow SMI depth, which were attributed to the high contributions of AOM-induced carbonate precipitation. Signatures of methanotrophic archaeal populations within the sedimentary buried nodule were detected based on microbial community composition analyses and quantitative real-time PCR targeted 16S rRNA, and functional genes for AOM. These results suggest that the buried carbonate nodule currently develops AOM-related microbial communities, and grows depending on the continued AOM under high methane flux conditions.
- Subjects
CARBONATE minerals; CARBONATES; METHANE; MICROBIAL communities; CARBON isotopes; FLUX (Energy); ANALYTICAL geochemistry; HABITATS
- Publication
Geosciences (2076-3263), 2019, Vol 9, Issue 11, p463
- ISSN
2076-3263
- Publication type
Article
- DOI
10.3390/geosciences9110463