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- Title
Identification of Mycobacterium spp. isolated from snakehead,Channa striata (Fowler), and Siamese fighting fish,Betta splendens (Regan), using polymerase chain reaction–reverse cross blot hybridization (PCR–RCBH).
- Authors
Puttinaowarat, S; Thompson, K D; Kolk, A; Adams, A
- Abstract
A variety of methods have been used to identify Mycobacterium spp. isolated from snakehead and Siamese fighting fish, including biochemistry, mycolic acid profiles and antibody-based methods. However, these methods are unable to differentiate between different species of Mycobacterium . Polymerase chain reaction (PCR) followed by reverse cross blot hybridization (RCBH) was adapted in this study to speciate aquatic mycobacteria. The method was highly specific for Mycobacterium spp. and identified the bacteria to species level with a detection limit of 100 fg DNA, equivalent to 20 mycobacteria. Twenty-nine isolates previously collected and cultured from Siamese fighting fish (10 isolates) and snakehead (19 isolates) during outbreaks of mycobacteriosis were analysed using PCR–RCBH. Six of the Siamese fighting fish isolates and nine of the snakehead isolates were identified as Mycobacterium fortuitum , while the remainder were classified as M. marinum . Notably, two isolates recovered from snakehead and Siamese fighting fish, previously identified as M. poriferae and M. piscicida , respectively, were confirmed to be M. fortuitum .
- Subjects
MYCOBACTERIUM; POLYMERASE chain reaction; SPECIES hybridization
- Publication
Journal of Fish Diseases, 2002, Vol 25, Issue 4, p235
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1046/j.1365-2761.2002.00363.x