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- Title
Extended longevity and robust early-stage development of C aenorhabditis elegans by a soil microbe, L ysinibacillus sphaericus.
- Authors
Go, Junhyeok; Lee, Kang-Mu; Park, Yongjin; Yoon, Sang Sun
- Abstract
C aenorhabditis elegans, originally isolated from soil, is a nematode used in various fields of biological research including host-microbe interaction. While bacterial pathogens responsible for human infections have been actively studied in C . elegans, very few bacterial species that provide beneficial effects on C . elegans have been reported. Here, we tested several bacterial soil isolates and then characterized the effects of L ysinibacillus sphaericus on C . elegans growth-related phenotypes. Worms fed with L . sphaericus lived significantly longer than those growing with typical E scherichia coli OP50. Early- and juvenile-stage growth was also highly stimulated by L . sphaericus; body size at 28 h post-hatching was > 2 times larger than OP50-fed worms and L . sphaericus-fed worms moved through the larval stage development more rapidly than control worms. In addition, significantly elevated fertilization was observed in worms fed with L . sphaericus (∼ 8 h faster than the control group). Furthermore, growth with L . sphaericus resulted in the production of larger numbers of progeny than the control growth with OP50. Worms grown with L . sphaericus were highly resistant to oxidative, osmotic and infection stresses. Together, our results reveal a novel mode of growth that involves healthy ageing of nematodes.
- Subjects
CAENORHABDITIS elegans; SOIL microbiology; NEMATODE development; LONGEVITY; OXIDATIVE stress
- Publication
Environmental Microbiology Reports, 2014, Vol 6, Issue 6, p730
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/1758-2229.12196