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- Title
Distribution of <em>Porphyromonas gingivalis</em> and <em>Treponema denticola</em> in human subgingival plaque at different periodontal pocket depths examined by immunohistochemical methods.
- Authors
Kigure, T.; Saito, A.; Seida, K.; Yamada, S.; Ishihara, K.; Okuda, K.
- Abstract
Localization of <em>Porphyromonas gingivalis</em> and <em>Treponema denticola</em> in different areas of subgingival plaque from advanced adult periodontitis patients was studied immunohistochemically using sensitive immunogold-silver staining and immunoelectron microscopy'. Fourteen periodontally diseased teeth were extracted without damaging the subgingival plaque, fixed, and embedded. The subgingival plaque samples were sectioned according to four different pocket depths (0.2 mm. 2 -4 mm. 4 6 mm and ≥6 mm). Serial thin sections were stained using specific antisera to <em>P. gingivalis</em> or <em>T. denticola</em> and then with secondary antibody labelled with colloidal-gold. Cells of both <em>P. gingivalis</em> and <em>T. denticola</em> were predominantly found in subgingival plaque located at depths of more than 4 mm in periodontal pockets. <em>T. denticola</em> cells were found in the surface layers of subgingival plaque. and <em>P. gingivalis</em> were predominant beneath them. However, in the deeper subgingival plaque. the coexistence of <em>P. gingivalis</em> and <em>T. denticola</em> was observed. The present findings suggest that <em>P. gingivalis</em> and <em>T. denticola</em> play important roles in the pathogenicity of periodontal disease and provide the useful information for elucidating the pattern of colonization of microorganisms in the periodontal pocket.
- Subjects
PORPHYROMONAS; GINGIVAL fluid; TREPONEMA; PLAQUES &; plaquettes; PERIODONTAL disease; MEDICAL microscopy; MICROORGANISMS
- Publication
Journal of Periodontal Research, 1995, Vol 30, Issue 5, p332
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.1995.tb01284.x