We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies.
- Authors
Bréard, Emmanuel; Lara, Estelle; Comtet, Loïc; Viarouge, Cyril; Doceul, Virginie; Desprat, Alexandra; Vitour, Damien; Pozzi, Nathalie; Cay, Ann Brigitte; De Regge, Nick; Pourquier, Philippe; Schirrmeier, Horst; Hoffmann, Bernd; Beer, Martin; Sailleau, Corinne; Zientara, Stéphan
- Abstract
A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-flurorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.
- Subjects
NUCLEOCAPSIDS; ENZYME-linked immunosorbent assay; VIRUSES; SIMBU virus; BUNYAVIRUSES; IMMUNOENZYME technique
- Publication
PLoS ONE, 2013, Vol 8, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0053446