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- Title
Optogenetic Control of Transcription in Zebrafish.
- Authors
Hongtao Liu; Gomez, Gustavo; Lin, Sophia; Shuo Lin; Chentao Lin
- Abstract
Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.
- Subjects
PROTEIN-protein interactions; MEDICAL technology; YEAST; ZEBRA danio; PLANT proteins; CRYPTOCHROMES
- Publication
PLoS ONE, 2012, Vol 7, Issue 11, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0050738