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- Title
Fc-Epsilon-RI, the High Affinity IgE-Receptor, Is Robustly Expressed in the Upper Gastrointestinal Tract and Modulated by Mucosal Inflammation.
- Authors
Bannert, Christina; Bidmon-Fliegenschnee, Bettina; Stary, Georg; Hotzy, Florian; Stift, Judith; Nurko, Samuel; Szépfalusi, Zsolt; Fiebiger, Edda; Dehlink, Eleonora
- Abstract
Background: The role of the high affinity IgE receptor, FcϵRI, in IgE-mediated immune responses of the gastrointestinal (GI) mucosa is poorly understood. Currently, a detailed characterization of FcϵRI expression throughout the human gut is lacking. The aim of this study was to define the expression pattern of FcϵRI in the GI tract. Methods/Principal Findings: We compared FcϵRI expression in children with gastritis/esophagitis (n = 10), celiac disease (n = 10), inflammatory bowel disease (IBD) (n = 9), and normal mucosa (n = 5). The α-subunit of FcϵRI (FcϵRIa), detected by immunohistochemistry, was found on cells infiltrating the mucosa of the esophagus, the stomach, and the duodenum, but was rarely detected in more distal sections of the GI tract. Accordingly, quantitative RT-PCR analysis on esophagus, stomach, duodenum, colon, and rectum biopsies revealed that FcϵRIa and -β expression levels decreased towards the distal intestine. mRNA transcripts of the common Fc-receptor-γ chain were present in the entire GI mucosa. Double-immunofluorescence staining of esophageal specimens confirmed that FcϵRIα was expressed on intraepithelial mast cells and Langerhans cells. The mRNA expression levels of the α, β, and γ subunits of FcϵRI did not correlate with total serum IgE but were associated with mucosal inflammation. Conclusion/Significance: Our data define the upper GI tract as the main site for IgE-mediated immune activation via FcϵRI. Tissue mRNA levels of FcϵRIα are regulated by inflammatory conditions rather than serum IgE, indicating that FcϵRI might also play a role in pathologies other than allergy.
- Subjects
GASTROINTESTINAL system; MUCOUS membranes; MESSENGER RNA; INTESTINAL diseases; CLINICAL pathology; BLOOD plasma; ESOPHAGUS; INFLAMMATION
- Publication
PLoS ONE, 2012, Vol 7, Issue 7, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0042066