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- Title
Autoradiographic detection of kinin receptors in the human medulla of control, hypertensive, and diabetic donors.
- Authors
Buck, Hudson de Sousa; Ongali, Brice; Thibault, Gaetan; Lindsey, Charles J.; Couture, Rejean
- Abstract
Kinins have been elected to the status of central neuromediators. Their effects are mediated through the activation of two G-protein-coupled receptors, denoted B[sub1] and B[sub2] . Functional and binding studies suggested that B[sub1] and B[sub2] receptors are upregulated in the medulla and spinal cord of hypertensive and diabetic rats. The aim of this study was to localize and quantify kinin receptors in post-mortem human medulla obtained from normotensive, hypertensive, and diabetic subjects, using in vitro receptor autoradiography with the radioligands [[sup125]I]HPP-HOE140 (B[sub2] receptor) and [[sup125]I]HPP[des-Arg[sup10]]-HOE140 (B[sub1] receptor). Data showed specific binding sites for B[sub2] receptor (0.4-1.5 fmol/mg tissue) in 11 medullary nuclei from 4 control specimens (paratrigeminal > ambiguus > cuneate, gelatinous layer of the caudal spinal trigeminal nucleus > caudal and interpolar spinal trigeminal, external cuneate, solitary tract > hypoglossal > gracile > inferior olivary nuclei). Increased density of B[sub2] receptor binding sites was observed in seven medullary nuclei of four hypertensive specimens (paratrigeminal > external cuneate > interpolar and caudal spinal trigeminal, gracile, inferior olivary > hypoglossal nuclei). B[sub2] receptor binding sites were seemingly increased in the same medullary nuclei of two diabetic specimens. Specific binding sites for B[sub1] receptor (1.05 and 1.36 fmol/mg tissue) were seen only in the inferior olivary nucleus in two out of the ten studied specimens. The present results support a putative role for kinins in the regulation of autonomic, nociceptive, and motor functions at the level of the human medulla. Evidence is also provided that B[sub2] receptors are upregulated in medullary cardiovascular...
- Subjects
KININS; ORGAN donors; SPINAL cord; MEDULLA oblongata; PEOPLE with diabetes
- Publication
Canadian Journal of Physiology & Pharmacology, 2002, Vol 80, Issue 4, p249
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/y02-050