We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Aldehyde dehydrogenase 1 expression in primary and metastatic renal cell carcinoma: an immunohistochemistry study.
- Authors
Abourbih, Samuel; Sircar, Kanishka; Tanguay, Simon; Kassouf, Wassim; Aprikian, Armen; Mansure, Jose; Brimo, Fadi
- Abstract
Background ALDH1 has been shown to be a cancer stem cell marker, and its expression correlates with prognosis in a number of malignancies. We aimed to evaluate to the expression of ALDH1 in a cohort of primary and metastatic RCC specimens, and to correlate expression with pathological outcomes such as tumor stage and grade, and clinical outcomes such as progression free survival. Methods Three tissue microarrays were constructed from 244 RCC specimens, taken from 1985 to 2006. Samples were stained using an ALDH1 monoclonal antibody and expression was quantified by degree of staining. Membrane and cytoplasm staining were considered separately. A retrospective chart review enabled correlation with clinical outcomes. Results ALDH1 expression did not vary significantly based on tumor stage (P = 0.6274) or grade (P = 0.1666). ALDH1 showed significantly more membranous expression in clear cell RCC versus other subtypes (P < 0.0001), as well as in the primary setting compared to metastases (P = 0.0216). In terms of progression free survival, no significant differences were seen based on ALDH1 expression levels. In a subanalysis of clear cell tumors, ALDH1 membranous expression was decreased in tumors of higher stage (P = 0.0233). Conclusions ALDH1 may be useful in characterizing RCC tumors as clear cell subtype. However, unlike in other malignancies, ALDH1 may not be useful in prognosticating renal cancers. The clinical significance of decreased ALDH1 expression in the high stage and metastatic setting remains to be determined in further investigations.
- Subjects
ALDEHYDE dehydrogenase; RENAL cell carcinoma; METASTASIS; GENE expression; STEM cells; CANCER prognosis
- Publication
World Journal of Surgical Oncology, 2013, Vol 11, Issue 1, p1
- ISSN
1477-7819
- Publication type
Article
- DOI
10.1186/1477-7819-11-298