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- Title
A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei industrial strain RUT-C30.
- Authors
Zhang, Lei; Zhang, Suping; Jiang, Xin; Wei, Wei; Wang, Wei; Wei, Dongzhi
- Abstract
Objectives: To develop a host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei (teleomorph Hypocrea jecorina) industrial strain RUT-C30. Results: The co-expression and purification system is based on: (i) an efficient and reliable selectable marker using bar (phosphinothricin acetyltransferase gene); (ii) a compact hygromycin B resistance marker; and (iii) a versatile integration plasmid for gene expression and knockout. Fluorescent protein genes were introduced into T. reesei and the corresponding proteins were purified from fermentation broth. The host-vector system was used in a proof-of-principle approach to achieve the co-expression of an alkaline endoglucanase and an alkaline cellobiohydrolase. Conclusions: This protocol can be used at an industrial scale to produce large amounts of proteins in T. reesei.
- Subjects
TRICHODERMA reesei; PROTEIN expression; FLUORESCENT proteins; HOST-parasite relationships; CELLULOSE 1,4-beta-cellobiosidase; GLUFOSINATE
- Publication
Biotechnology Letters, 2016, Vol 38, Issue 1, p89
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-015-1948-4