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- Title
A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization.
- Authors
Xiaoyan Suo; Xiuling Lu; Tao Hu; Guanghui Ma; Zhiguo Su
- Abstract
A solid-phase adsorption method was developed to circumvent the disadvantage of the conventional liquid-phase PEGylation, i.e. the heterogeneity of the PEGylated products. The model proteins, human serum albumin (HSA) and staphylokinase (SAK), were adsorbed on the ion exchange chromatography media, followed by PEGylation with succinimidyl carbonate (SC)-mPEG5K and salt elution. Since PEGylation with SC-PEG5K alters the positive charge of the proteins, Q-Sepharose Big Beads and DEAE Sepharose Fast Flow were used for adsorption of HSA and SAK, respectively. Size exclusion chromatography and SDS-PAGE studies demonstrated that solid-phase PEGylation of proteins generate monoPEGylated proteins with the yield of 35–47%. Circular dichroism and intrinsic fluorescence studies showed that solid-phase PEGylation led to little conformational change of the proteins. Solid-phase PEGylation resulted in 35% loss in the biological activity of SAK, which is lower than the liquid-phase PEGylation (70%).
- Subjects
ADSORPTION (Chemistry); SERUM albumin; BLOOD proteins; ION exchange (Chemistry); BIOMOLECULES
- Publication
Biotechnology Letters, 2009, Vol 31, Issue 8, p1191
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-009-9986-4