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- Title
No association of estrogen receptor a and cytochrome P450c17a polymorphisms with age at menopause in a Dutch cohort.
- Authors
Helen S. Kok; N. Charlotte Onland-Moret; Kristel M. van Asselt; Carla H. van Gils; Yvonne T. van der Schouw; Diederick E. Grobbee; Petra H.M. Peeters
- Abstract
BACKGROUND: Age at menopause is under strong genetic control. So far, genetic variations of only one gene, the PvuII polymorphism of the estrogen receptor a (ERa) gene, have been shown to be associated with age at onset of menopause. This study aims to investigate whether PvuII, XbaI and B-variant polymorphisms of the ERa gene, and the MspAI polymorphism of the cytochrome P450c17a (CYP17) gene are associated with age at menopause in a Dutch cohort. METHODS: DNA was isolated from urine samples of 385 Caucasian women with natural menopause and the genotypes of the four polymorphisms were determined. A questionnaire was used for background characteristics. The genotypes of PvuII, XbaI, MspAI were obtained by PCR restriction fragment length polymorphism analysis. The B-variant was determined with an allele-specific oligonucleotide hybridization method. Two-sided t-tests were performed to assess the association between the four polymorphisms and menopausal age. The PvuII and XbaI polymorphisms were analysed separately as well as in a combined score. RESULTS: The results show that none of the polymorphisms independently, nor the combined genotypes for PvuII and XbaI, were associated with age at natural menopause. CONCLUSION: No evidence was found for a relationship between common variants of the ERa gene and the CYP17 gene with age at natural menopause.
- Subjects
MENOPAUSE; CLIMACTERIC; SEXUAL cycle; CYTOCHROMES
- Publication
Human Reproduction, 2005, Vol 20, Issue 2, p536
- ISSN
0268-1161
- Publication type
Article
- DOI
10.1093/humrep/deh600