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- Title
Reversal of multidrug resistance of vincristine-resistant gastric adenocarcinoma cells through up-regulation of DARPP-32
- Authors
Hong, Liu; Wang, Jin; Han, Ying; Zhao, Yunping; Gao, Juan; Wang, Jun; Han, Yu; Zhang, Xiaoyin; Yan, Li; Zhou, Xinmin; Qiao, Taidong; Chen, Zhen; Fan, Daiming
- Abstract
Abstract: Here we investigated the roles of DARPP-32 in multidrug resistance (MDR) of gastric cancer cells and the possible underlying mechanisms. We constructed the eukaryotic expression vector of DARPP-32 and transfected it into human vincristine-resistant gastric adenocarcinoma cell line SGC7901/VCR. Up-regulation of DARPP-32 could significantly enhance the sensitivity of SGC7901/VCR cells towards vincristine, adriamycin, 5-fluorouracil and cisplatin, and could decrease the capacity of cells to efflux adriamycin. What''s more, the results of subrenal capsule assay confirmed that DARPP-32 might play a certain role in MDR of gastric cancer. DARPP-32 could significantly down-regulate the expression of P-gp and zinc ribbon domain-containing 1 (ZNRD1), but not alter the expression of multidrug resistance-associated protein (MRP) or the glutathione S-transferase (GST). DARPP-32 could also significantly decrease the anti-apoptotic activity of SGC7901/VCR cells. Further study of the biological functions of DARPP-32 might be helpful for understanding the mechanisms of MDR in gastric cancer.
- Subjects
DRUG resistance; ANTINEOPLASTIC agents; CANCER cells; GENETIC vectors
- Publication
Cell Biology International, 2007, Vol 31, Issue 9, p1010
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2007.03.020