We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Development of 14 EST-SSRs for Betula maximowicziana and their applicability to related species.
- Authors
Tsuda, Yoshiaki; Ueno, Saneyoshi; Ide, Yuji; Tsumura, Yoshihiko
- Abstract
Simple sequence repeats (SSRs) markers were developed for Betula maximowicziana using 2698 expressed sequence tags (ESTs) from the NCBI database. Out of 112 designed primer pairs, 54 showed clear PCR amplification and 14 of these revealed polymorphism in eight individuals sampled across the species' range. The number of alleles detected and the expected heterozygosity ranged from 1 to 3 and 0.000 to 0.570, respectively, when these 14 loci were examined in 49 individuals from a single population. In the cross species transferability test, eight of the 14 loci were also polymorphic in all four of the diploid, tetraploid and hexaploid Betula species examined. These results showed high transferability of the developed EST-SSRs and that these markers are likely to be useful in studies of the population genetics of species in the genus Betula.
- Subjects
SCHISANDRA; BIRCH; MICROSATELLITE repeats; GENE amplification; GENETIC polymorphisms; POPULATION genetics
- Publication
Conservation Genetics, 2009, Vol 10, Issue 3, p661
- ISSN
1566-0621
- Publication type
Article
- DOI
10.1007/s10592-008-9608-z