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- Title
Machilin D, a Lignin Derived from Saururus chinensis, Suppresses Breast Cancer Stem Cells and Inhibits NF-κB Signaling.
- Authors
Zhen, Xing; Choi, Hack Sun; Kim, Ji-Hyang; Kim, Su-Lim; Liu, Ren; Yun, Bong-Sik; Lee, Dong-Sun
- Abstract
Cancer stem cells are responsible for breast cancer initiation, metastasis, and relapse. Targeting breast cancer stem cells (BCSCs) using phytochemicals is a good strategy for the treatment of cancer. A silica gel, a reversed-phase C18 column (ODS), a Sephadex LH-20 gel, thin layer chromatography, and high-performance liquid chromatography (HPLC) were used for compound isolation from Saururus chinensis extracts. The isolated compound was identified as machilin D by mass spectrometry and nuclear magnetic resonance (NMR). Machilin D inhibited the growth and mammosphere formation of breast cancer cells and inhibited tumor growth in a xenograft mouse model. Machilin D reduced the proportions of CD44+/CD24- and aldehyde dehydrogenase 1 (ALDH1)-positive cells. Furthermore, this compound reduced the nuclear localization of the NF-κB protein and decreased the IL-6 and IL-8 secretion in mammospheres. These results suggest that machilin D blocks IL-6 and IL-8 signaling and induces CSC death and thus may be a potential agent targeting BCSCs.
- Subjects
CANCER stem cells; BREAST cancer; THIN layer chromatography; HIGH performance liquid chromatography; ALDEHYDE dehydrogenase; PHYTOCHEMICALS; SILICA gel
- Publication
Biomolecules (2218-273X), 2020, Vol 10, Issue 2, p245
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom10020245