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- Title
Anomalous Subdiffusion of Calcium Spark in Cardiac Myocytes.
- Authors
Li, Kaitao; Fu, Chaoqi; Cheng, Heping; Tan, Wenchang
- Abstract
'Ca spark' is the elementary event of calcium release through ryanodine receptors (RyRs) from sarcoplasmic reticulum (SR) during the excitation-contraction (EC) coupling process in cardiac myocytes. Questions remain about its underlying physical process, and existing Fick diffusion-based models fail to reproduce the morphology of experimentally recorded Ca sparks, a phenomenon known as the full-width at half maximum (FWHM) paradox. Here we developed an anomalous subdiffusion model involving Ca release from SR, cytosolic diffusion, recycling by SR Ca-ATPases, and the interaction with Ca-binding sites (including the diffusible Ca indicator). Numerically simulated Ca spark fit perfectly with experimental results in terms of morphology and kinetics, suggesting the anomalous space subdiffusion to be the governing process of Ca diffusion in the cytoplasm. In addition, parameter sensitivity analysis revealed the essential determinants of spark morphology to be the Ca diffusivity, reaction kinetics, and indicator concentration, but the stationary buffers and pumps to a less extent.
- Subjects
CALCIUM; SARCOPLASMIC reticulum; ORGANELLES; MUSCLE cells; CYTOPLASM
- Publication
Cellular & Molecular Bioengineering, 2011, Vol 4, Issue 3, p457
- ISSN
1865-5025
- Publication type
Article
- DOI
10.1007/s12195-011-0174-y