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- Title
Purification and Characterization of a Novel Isoallergen of a Major Bermuda Grass Pollen Allergen, Cyn d 1.
- Authors
Song-Nan Su, George C.; Ho-Jen Peng, George C.; Sue-Yee Yang, George C.; Lai-Chen Tsai; Lu-Ping Chow; Shih-Wen Huang
- Abstract
A novel immunoreactive isoallergen of a major Bermuda grass pollen allergen, Cyn d 1, was purified by the use of a combination of various chromatographic techniques, including high-performance liquid chromatography. This new isoallergen has a pI value of 9.1 and shows significant N-terminal sequence homology with other isoforms. Carbohydrate composition analysis revealed a 10.4% carbohydrate content consisting of 7 different sugar moieties, including arabinose, fucose, galactose, glucose, mannose, xylose and N-acetylglucosamine, as well as a trace amount of rhamnose. Upon periodate oxidation, the binding activities of the Cyn d 1 isoform to murine monoclonal antibodies and human serum IgE and IgG were reduced, suggesting the importance of the carbohydrate moiety in the immune response. The availability of the purified Cyn d 1 basic isoform will allow for further structural and immunological characterization, and ultimately for the design of an appropriate therapy.Copyright © 2003 National Science Council, ROC and S. Karger AG, Basel
- Subjects
BERMUDA grass; POLLEN; ALLERGENS; AIR purification; CARBOHYDRATES
- Publication
Journal of Biomedical Science, 2003, Vol 10, Issue 1, p111
- ISSN
1021-7770
- Publication type
Article
- DOI
10.1007/BF02256003