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- Title
Comparative Analysis of Leucocyte Count (Total and Differential) in Patients with Leucocytosis using Sysmex XN550-L Series (5 Part) Automated Analyzer and Conventional Manual Technique in a Tertiary Care Hospital in Rural Haryana.
- Authors
Sharma, Abhimanyu; Isha; Lone, Aasif Hamid; Kaur, Rupinder; Nijhawan, Vijay S.
- Abstract
Normal concentration of WBCs in blood varies between 4000 and 11,000 per microliter and are classified into two variants namely granulocytes and agranulocytes on the basis of presence and absence of granules. Manual methods of calculation of TLC include- Neubauer chamber and estimating WBC on leishman stained slides whereas automated methods are based on impedance or light scattering technology. The study compared the efficacy of both these methods in statistical terms using Chi Square test. 100 cases of leucocytosis analyzed over a period of 1 year at MMIMSR, Mullana, Haryana revealed leucocytosis prevalent in 4-6 decade of life with slight male predominance. The pearson coefficient for TLC came out to be p value =0.215 (not significant) but for DLC, calculations revealed p values for polymorphs, lymphocytes, eosinophils and monocytes to be significant (p value =0.000 each). To conclude, standard manual methods should be advocated as special practice where there is definite need of morphological and quantitative evaluation of WBCs and to validate the automated methods by which the laboratories can optimize patient care and not as a replacement.
- Subjects
HARYANA (India); LEUCOCYTOSIS; RURAL hospitals; HOSPITAL care; TERTIARY care; CHI-squared test
- Publication
JK Science, 2020, Vol 22, Issue 2, p61
- ISSN
0972-1177
- Publication type
Article