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- Title
Parenteral Soybean Oil Induces Hepatosteatosis Despite Addition of Fish Oil in a Mouse Model of Intestinal Failure-Associated Liver Disease.
- Authors
Nandivada, Prathima; Fell, Gillian L.; Pan, Amy H.; Nose, Vania; Mitchell, Paul D.; Gura, Kathleen M.; Puder, Mark
- Abstract
<bold>Background: </bold>Replacement of parenteral soybean oil (SO) with fish oil (FO) is an effective therapy for intestinal failure-associated liver disease (IFALD) in children. However, practitioners remain concerned about the risk of essential fatty acid deficiency (EFAD) and sometimes treat IFALD with a combination of 50% SO and 50% FO emulsions. The purpose of this study was to determine if mixing 50% SO and 50% FO emulsions would prevent hepatosteatosis in a murine model of parenteral nutrition (PN)-induced hepatosteatosis.<bold>Methods: </bold>C57BL/6 mice were randomized to receive oral PN with parenteral saline, FO, SO, or a mixture of 50% FO and 50% SO for 19 days. Fatty acid analysis, histologic evaluation, Nonalcoholic Steatohepatitis Clinical Research Network (NSCRN) scores, and reverse-transcriptase polymerase chain reaction for key lipogenic genes were performed.<bold>Results: </bold>The PN + saline group was the only group with EFAD, with a serum and hepatic triene/tetraene ratio of 0.53. NSCRN scores were highest in the PN + SO group (5.5; 95% confidence interval [CI], 4.9-6.1), followed by the PN + FO/SO (4.5; 95% CI, 3.5-5.5) group, with the lowest score in the PN + FO (2.0; 95% CI, 1.1-2.9) group. Acetyl CoA carboxylase α and acetyl CoA carboxylase β expression was lower in the PN + FO group than in the PN + FO/SO or PN + SO groups.<bold>Conclusions: </bold>Our data demonstrate that a mixed fat emulsion of 50% SO and 50% FO is inferior to 100% FO in reducing hepatosteatosis in this model. These data suggest that use of parenteral SO with parenteral FO, in a 1:1 ratio, may still contribute to liver injury, although it is less hepatotoxic than pure SO.
- Subjects
FISH oils; INTRAVENOUS fat emulsions; FATTY acid analysis; ENZYME metabolism; ANIMAL experimentation; CONFIDENCE intervals; FATTY liver; INTESTINAL diseases; LIVER diseases; MICE; PARENTERAL feeding; POLYMERASE chain reaction; SOY oil; TREATMENT effectiveness; REVERSE transcriptase polymerase chain reaction; DISEASE complications; THERAPEUTICS
- Publication
JPEN Journal of Parenteral & Enteral Nutrition, 2018, Vol 42, Issue 2, p403
- ISSN
0148-6071
- Publication type
journal article
- DOI
10.1177/0148607117695249