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- Title
Safe sequencing depth to estimate the intra-host heterogeneity of viruses.
- Authors
Xianwen Ren; Qi Jin
- Abstract
Massively parallel sequencing allows efficient determination of genomic sequences and intra-host polymorphisms for viruses. However, the sequencing depth that guarantees safe interpretation of the observed data is unclear. We demonstrated that 10-fold genome coverage may allow safe genome assembly, and 1000-fold coverage is required to obtain reliable polymorphism estimation.
- Subjects
EBOLA virus disease; GENETIC polymorphisms; NUCLEOTIDE sequence; VIRAL genomes; HOST-virus relationships; GENETICS
- Publication
Briefings in Functional Genomics, 2016, Vol 15, Issue 4, p275
- ISSN
2041-2649
- Publication type
Article
- DOI
10.1093/bfgp/elv039