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- Title
High-speed coherent Raman fingerprint imaging of biological tissues.
- Authors
Camp Jr, Charles H.; Lee, Young Jong; Heddleston, John M.; Hartshorn, Christopher M.; Walker, Angela R. Hight; Rich, Jeremy N.; Lathia, Justin D.; Cicerone, Marcus T.
- Abstract
An imaging platform based on broadband coherent anti-Stokes Raman scattering has been developed that provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically relevant Raman window (500-3,500 cm-1) with high resolution (<10 cm-1). It strongly and efficiently stimulates Raman transitions within the typically weak 'fingerprint' region using intrapulse three-colour excitation, and utilizes the non-resonant background to heterodyne-amplify weak Raman signals. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues as well as interfaces between xenograft brain tumours and the surrounding healthy brain matter.
- Subjects
ANTI-Stokes scattering; IMAGING systems in chemistry; LABORATORY mice; XENOGRAFTS; BRAIN tumors
- Publication
Nature Photonics, 2014, Vol 8, Issue 8, p627
- ISSN
1749-4885
- Publication type
Article
- DOI
10.1038/nphoton.2014.145