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- Title
Recent progress in mass spectrometry-based strategies for elucidating protein–protein interactions.
- Authors
Low, Teck Yew; Syafruddin, Saiful Effendi; Mohtar, M. Aiman; Vellaichamy, Adaikkalam; A Rahman, Nisa Syakila; Pung, Yuh-Fen; Tan, Chris Soon Heng
- Abstract
Protein–protein interactions are fundamental to various aspects of cell biology with many protein complexes participating in numerous fundamental biological processes such as transcription, translation and cell cycle. MS-based proteomics techniques are routinely applied for characterising the interactome, such as affinity purification coupled to mass spectrometry that has been used to selectively enrich and identify interacting partners of a bait protein. In recent years, many orthogonal MS-based techniques and approaches have surfaced including proximity-dependent labelling of neighbouring proteins, chemical cross-linking of two interacting proteins, as well as inferring PPIs from the co-behaviour of proteins such as the co-fractionating profiles and the thermal solubility profiles of proteins. This review discusses the underlying principles, advantages, limitations and experimental considerations of these emerging techniques. In addition, a brief account on how MS-based techniques are used to investigate the structural and functional properties of protein complexes, including their topology, stoichiometry, copy number and dynamics, are discussed.
- Subjects
PROTEIN-protein interactions; MASS spectrometry; CELL cycle; CYTOLOGY
- Publication
Cellular & Molecular Life Sciences, 2021, Vol 78, Issue 13, p5325
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-021-03856-0