We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Engineered nanostructured β-sheet peptides protect membrane proteins.
- Authors
Tao, Houchao; Lee, Sung Chang; Moeller, Arne; Roy, Rituparna Sinha; Siu, Fai Yiu; Zimmermann, Jörg; Stevens, Raymond C; Potter, Clinton S; Carragher, Bridget; Zhang, Qinghai
- Abstract
We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.
- Subjects
NANOSTRUCTURED materials; PEPTIDES; MEMBRANE proteins; MOLECULAR self-assembly; ATP-binding cassette transporters; CLUSTERING of particles
- Publication
Nature Methods, 2013, Vol 10, Issue 8, p759
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2533