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- Title
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.
- Authors
Venken, Koen J. T.; Schulze, Karen L.; Haelterman, Nele A.; Hongling Pan; Yuchun He; Evans-Holm, Martha; Carlson, Joseph W.; Levis, Robert W.; Spradling, Allan C.; Hoskins, Roger A.; Bellen, Hugo J.
- Abstract
We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow+ marker flanked by two inverted bacteriophage ?C31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNA manipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit.
- Subjects
DROSOPHILA melanogaster; TRANSPOSONS; BACTERIOPHAGES; GENE expression; GENOMES
- Publication
Nature Methods, 2011, Vol 8, Issue 9, p737
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1662