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- Title
The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach.
- Authors
van Leeuwen, S. J. M.; Proctor, G. B.; Staes, A.; Laheij, A. M. G. A.; Potting, C. M. J.; Brennan, M. T.; von Bültzingslöwen, I.; Rozema, F. R.; Hazenberg, M. D.; Blijlevens, N. M. A.; Raber-Durlacher, J. E.; Huysmans, M. C. D. N. J. M.
- Abstract
Background: Oral mucositis is a frequently seen complication in the first weeks after hematopoietic stem cell transplantation recipients which can severely affects patients quality of life. In this study, a labelled and label-free proteomics approach were used to identify differences between the salivary proteomes of autologous hematopoietic stem cell transplantation (ASCT) recipients developing ulcerative oral mucositis (ULC-OM; WHO score ≥ 2) or not (NON-OM). Methods: In the TMT-labelled analysis we pooled saliva samples from 5 ULC-OM patients at each of 5 timepoints: baseline, 1, 2, 3 weeks and 3 months after ASCT and compared these with pooled samples from 5 NON-OM patients. For the label-free analysis we analyzed saliva samples from 9 ULC-OM and 10 NON-OM patients at 6 different timepoints (including 12 months after ASCT) with Data-Independent Acquisition (DIA). As spectral library, all samples were grouped (ULC-OM vs NON-OM) and analyzed with Data Dependent Analysis (DDA). PCA plots and a volcano plot were generated in RStudio and differently regulated proteins were analyzed using GO analysis with g:Profiler. Results: A different clustering of ULC-OM pools was found at baseline, weeks 2 and 3 after ASCT with TMT-labelled analysis. Using label-free analysis, week 1–3 samples clustered distinctly from the other timepoints. Unique and up-regulated proteins in the NON-OM group (DDA analysis) were involved in immune system-related processes, while those proteins in the ULC-OM group were intracellular proteins indicating cell lysis. Conclusions: The salivary proteome in ASCT recipients has a tissue protective or tissue-damage signature, that corresponded with the absence or presence of ulcerative oral mucositis, respectively. Trial registration: The study is registered in the national trial register (NTR5760; automatically added to the International Clinical Trial Registry Platform).
- Subjects
PROTEIN analysis; SALIVA analysis; STOMATITIS; AUTOTRANSFUSION of blood; IMMUNE system; PROTEOMICS; QUALITY of life; RESEARCH funding; HEMATOPOIETIC stem cell transplantation; MULTIPLE myeloma; DISEASE risk factors
- Publication
BMC Oral Health, 2023, Vol 23, Issue 1, p1
- ISSN
1472-6831
- Publication type
Article
- DOI
10.1186/s12903-023-03190-w