We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Metabolic pathway that produces essential fatty acids from polymethylene-interrupted polyunsaturated fatty acids in animal cells.
- Authors
Tanaka, Tamotsu; Morishige, Jun-ichi; Iwawaki, Dai; Fukuhara, Terumi; Hamamura, Naomi; Hirano, Kaoru; Osumi, Takashi; Satouchi, Kiyoshi
- Abstract
Sciadonic acid (20:3 Δ-5,11,14) and juniperonic acid (20:4 Δ-5,11,14,17) are polyunsaturated fatty acids (PUFAs) that lack the Δ-8 double bond of arachidonic acid (20:4 Δ-5,8,11,14) and eicosapentaenoic acid (20:5 Δ-5,8,11,14,17), respectively. Here, we demonstrate that these conifer oil-derived PUFAs are metabolized to essential fatty acids in animal cells. When Swiss 3T3 cells were cultured with sciadonic acid, linoleic acid (18:2 Δ-9,12) accumulated in the cells to an extent dependent on the concentration of sciadonic acid. At the same time, a small amount of 16:2 Δ-7,10 appeared in the cellular lipids. Both 16:2 Δ-7,10 and linoleic acid accumulated in sciadonic acid-supplemented CHO cells, but not in peroxisome-deficient CHO cells. We confirmed that 16:2 Δ-7,10 was effectively elongated to linoleic acid in rat liver microsomes. These results indicate that sciadonic acid was partially degraded to 16:2 Δ-7,10 by two cycles of β-oxidation in peroxisomes, then elongated to linoleic acid in microsomes. Supplementation of Swiss 3T3 cells with juniperonic acid, an n−3 analogue of sciadonic acid, induced accumulation of α-linolenic acid (18:3 Δ-9,12,15) in cellular lipids, suggesting that juniperonic acid was metabolized in a similar manner to sciadonic acid. This PUFA remodeling is thought to be a process that converts unsuitable fatty acids into essential fatty acids required by animals.
- Subjects
ESSENTIAL fatty acids; UNSATURATED fatty acids; OXIDATION; CELL culture; PEROXISOMES; LIPIDS
- Publication
FEBS Journal, 2007, Vol 274, Issue 11, p2728
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2007.05807.x