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- Title
Age-determined expression of priming protease TMPRSS2 and localization of SARS-CoV-2 in lung epithelium.
- Authors
Schuler, Bryce A.; Habermann, A. Christian; Plosa, Erin J.; Taylor, Chase J.; Jetter, Christopher; Negretti, Nicholas M.; Kapp, Meghan E.; Benjamin, John T.; Gulleman, Peter; Nichols, David S.; Braunstein, Lior Z.; Hackett, Alice; Koval, Michael; Guttentag, Susan H.; Blackwell, Timothy S.; Webber, Steven A.; Banovich, Nicholas E.; Kropski, Jonathan A.; Sucre, Jennifer M. S.; Vanderbilt COVID-19 Consortium Cohort
- Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) novel coronavirus 2019 (COVID-19) global pandemic has led to millions of cases and hundreds of thousands of deaths. While older adults appear at high risk for severe disease, hospitalizations and deaths due to SARS-CoV-2 among children have been relatively rare. Integrating single-cell RNA sequencing (scRNA-seq) of developing mouse lung with temporally resolved immunofluorescence in mouse and human lung tissue, we found that expression of SARS-CoV-2 Spike protein primer TMPRSS2 was highest in ciliated cells and type I alveolar epithelial cells (AT1), and TMPRSS2 expression increased with aging in mice and humans. Analysis of autopsy tissue from fatal COVID-19 cases detected SARS-CoV-2 RNA most frequently in ciliated and secretory cells in airway epithelium and AT1 cells in peripheral lung. SARS-CoV-2 RNA was highly colocalized in cells expressing TMPRSS2. Together, these data demonstrate the cellular spectrum infected by SARS-CoV-2 in lung epithelium and suggest that developmental regulation of TMPRSS2 may underlie the relative protection of infants and children from severe respiratory illness.
- Subjects
SARS-CoV-2; COVID-19; EPITHELIUM; LUNGS; EPITHELIAL cells
- Publication
Journal of Clinical Investigation, 2021, Vol 131, Issue 1, p1
- ISSN
0021-9738
- Publication type
journal article
- DOI
10.1172/JCI140766