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- Title
FeO nanoparticles engineered for plasmid DNA delivery to Escherichia coli.
- Authors
Saei, Amir; Barzegari, Abolfazl; Majd, Mostafa; Asgari, Davoud; Omidi, Yadollah
- Abstract
Heat shock treatment is the most popular method for transformation of Escherichia coli. We have used 19-nm FeO nanoparticles for improving heat shock protocol. PGEM- T (3,000 bp) and pCAMBIA (8,428 bp) were used as test plasmids for transformation of competent E. coli cells (strains DH5α and Jm107) obtained from heat shock- and CaCl-treated bacteria. A combination of heat shock and FeO nanoparticles led to a significant increase (6-10 fold) in number of transformed colonies in comparison with heat shock alone. The percent increase in transformation efficiency was higher for larger pCAMBIA plasmids compared to PGEM- T. The transformation efficiency decreased in the absence of CaCl and increased by addition of glycerol to the bacterial culture.
- Subjects
IRON oxides; NANOPARTICLES; DNA; ESCHERICHIA coli; BACTERIAL cells; MOLECULAR biology
- Publication
Journal of Nanoparticle Research, 2014, Vol 16, Issue 8, p1
- ISSN
1388-0764
- Publication type
Article
- DOI
10.1007/s11051-014-2521-0