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- Title
Adiabatic TOBSY in rotating solids.
- Authors
Leppert, Jörg; Ohlenschläger, Oliver; Görlach, Matthias; Ramachandran, Ramadurai
- Abstract
A MAS solid state NMR approach for achieving efficient scalar coupling mediated through-bond 13C chemical shift correlations of the aliphatic carbons in uniformly labelled peptides/proteins is described. The method involves the application of a continuous train of adiabatic inversion pulses, as in the adiabatic TOCSY experiments carried out in solution state NMR studies. While rotor synchronised application of adiabatic inversion pulses leads to dipolar correlations, it is shown here via numerical simulations and experimental measurements that asynchronous application of adiabatic pulses can facilitate the mapping of through-bond connectivities. The method employs a suitable phasing scheme for generating the desired isotropic mixing Hamiltonian and requires moderate 13C RF field strength only.
- Subjects
SOLIDS; PROTEINS; CHEMICAL structure; PEPTIDES; CARBON; HAMILTONIAN systems
- Publication
Journal of Biomolecular NMR, 2004, Vol 29, Issue 2, p167
- ISSN
0925-2738
- Publication type
Article
- DOI
10.1023/B:JNMR.0000019248.48726.ff