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- Title
Further investigation of the epitope recognized by the new monoclonal antibody 2C9.
- Authors
KATO, KEITARO; YOSHIKAWA, KAZUHIRO; TAKI, TOMOHIRO; SHITARA, KENYA; NAKAMURA, KAZUYASU; HIROTA, MAIKO; HANAI, NOBUO; NAKAMURA, KOGENTA; KOKUBO, HIROTO; MITSUI, KENJI; YAMADA, YOSHIAKI; HONDA, NOBUAKI; UEDA, RYUZO; SAGA, SHINSUKE; FUKATSU, HIDETOSHI
- Abstract
Abstract Objective: We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody. Methods: The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system. Results: The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA. Conclusion: The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.
- Subjects
MONOCLONAL antibodies; ANTIGENS; AMINO acid sequence
- Publication
International Journal of Urology, 2003, Vol 10, Issue 8, p439
- ISSN
0919-8172
- Publication type
Article
- DOI
10.1046/j.1442-2042.2003.00655.x