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- Title
Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells.
- Authors
Bonnet, Clémence; Oh, Denise; Mei, Hua; Robertson, Sarah; Chang, Derek; Bourges, Jean-Louis; Behar-Cohen, Francine; Zheng, Jie J.; Deng, Sophie X.
- Abstract
The corneal epithelium is consistently regenerated by limbal stem/progenitor cells (LSCs), a very small population of adult stem cells residing in the limbus. Several Wnt ligands, including Wnt6, are preferentially expressed in the limbus. To investigate the role of Wnt6 in regulating proliferation and maintenance of human LSCs in an in vitro LSC expansion setting, we generated NIH-3T3 feeder cells to overexpress different levels of Wnt6. Characterization of LSCs cultured on Wnt6 expressing 3T3 cells showed that high level of Wnt6 increased proliferation of LSCs. Medium and high levels of Wnt6 also increased the percentage of small cells (diameter ≤ 12 µm), a feature of the stem cell population. Additionally, the percentage of cells expressing the differentiation marker K12 was significantly reduced in the presence of medium and high Wnt6 levels. Although Wnt6 is mostly known as a canonical Wnt ligand, our data showed that canonical and non-canonical Wnt signaling pathways were activated in the Wnt6-supplemented LSC cultures, a finding suggesting that interrelationships between both pathways are required for LSC regulation.
- Subjects
NATIONAL Institutes of Health (U.S.); PROGENITOR cells; CELLULAR signal transduction; CELL populations; CELL differentiation; STEM cells; WNT signal transduction
- Publication
Scientific Reports, 2021, Vol 11, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-021-00273-y