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- Title
A PLCγ1-Dependent, Force-Sensitive Signaling Network in the Myogenic Constriction of Cerebral Arteries.
- Authors
Gonzales, Albert L.; Ying Yang; Sullivan, Michelle N.; Sanders, Lindsey; Dabertrand, Fabrice; Hill-Eubanks, David C.; Nelson, Mark T.; Earley, Scott
- Abstract
Maintaining constant blood flow in the face of fluctuations in blood pressure is a critical autoregulatory feature of cerebral arteries. An increase in pressure within the artery lumen causes the vessel to constrict through depolarization and contraction of the encircling smooth muscle cells. This pressure-sensing mechanism involves activation of two types of transient receptor potential (TRP) channels: TRPC6 and TRPM4. We provide evidence that the activation of the γ1 isoform of phospholipase C (PLCγ1) is critical for pressure sensing in cerebral arteries. Inositol 1,4,5-trisphosphate (IP3), generated by PLCγ1 in response to pressure, sensitized IP3 receptors (IP3Rs) to Ca2+ influx mediated by the mechanosensitive TRPC6 channel, synergistically increasing IP3R-mediated Ca2+ release to activate TRPM4 currents, leading to smooth muscle depolarization and constriction of isolated cerebral arteries. Proximity ligation assays demonstrated colocalization of PLCγ1 and TRPC6 with TRPM4, suggesting the presence of a force-sensitive, local signaling network comprisingPLCγ1, TRPC6, TRPM4, and IP3Rs.Src tyrosine kinase activitywas necessary for stretch-induced TRPM4 activation andmyogenic constriction, consistent with the ability of Src toactivate PLCγ isoforms. We conclude that contraction of cerebral artery smoothmuscle cells requires the integration of pressure-sensing signaling pathways and their convergence on IP3Rs, which mediate localized Ca2+-dependent depolarization through the activation of TRPM4.
- Publication
Science Signaling, 2014, Vol 7, Issue 327, p1
- ISSN
1945-0877
- Publication type
Article
- DOI
10.1126/scisignal.2004732