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- Title
A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b.
- Authors
Sun, Wenyang; Lan, Jing; Chen, Lei; Qiu, Jinjie; Luo, Zonggang; Li, Mingzhou; Wang, Jinyong; Zhao, Jiugang; Zhang, Tinghuan; Long, Xi; Chai, Jie; Yan, Zunqiang; Guo, Zongyi; Gun, Shuangbao
- Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that are involved in translational regulation of the messenger RNA molecules. Sequence variations in the genes encoding miRNAs could influence their biogenesis and function. MiR-15b plays an important role in cellular proliferation, apoptosis and the cell cycle. Here, we report the identification of a C58T mutation in porcine pre-miR-15b. Through in vitro and in vivo experiments, we determined that this mutation blocks the transition from pri-miRNA to pre-miRNA, alters the strand selection between miR-15b-5p and miR-15b-3p, and obstructs biogenesis of the downstream miR-16-1. These results serve to highlight the importance of miRNA mutations and their impacts on miRNA biogenesis.
- Subjects
MICRORNA; ORIGIN of life; CELL proliferation; CELL cycle; GENETIC translation
- Publication
PLoS ONE, 2017, Vol 12, Issue 5, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0178045