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- Title
SEARCH FOR NATURAL AND NATURAL-LIKE INHIBITORS OF TRICHOTHECENE BIOSYNTHESIS BY FUSARIUM.
- Authors
Pani, G.; Balmas, V.; Scherm, B.; Marcello, A.; Fabbri, D.; Dettori, M. A.; Azara, E.; Dessì, A.; Dallocchio, R.; Delogu, G.; Migheli, Q.
- Abstract
Fusarium culmorum is a major fungal pathogen of wheat, causing crown and foot rot (CFR) and Fusarium head blight (FHB). Yield losses occur for the grain becomes contaminated by mycotoxins. Among the most bioactive compounds, trichothecenes are able to inhibit eukaryotic protein synthesis and cause toxicoses to humans or animals consuming contaminated food or feed. Trichothecenes induce apoptosis and play an important role in the aggressiveness of phytopathogenic Fusarium species towards plant hosts. The aim of this project was to design, prepare and study new natural and natural-like compounds to be applied in the control of F. culmorum mycotoxin production. Particular attention was paid to the selection and preparation of compounds with selective trichothecene B inhibitory activity compared to compounds showing both mycotoxin inhibitory and fungitoxic activities. The first inhibition experiments were performed using compounds belonging to the family of gallic acid, phenylpropanoids and cinnamic derived acids. In vivo and in vitro tests and molecular modeling with computational studies were carried out. A straightforward thin layer chromatography (TLC) method and a quantitative LC-MS analysis were used to identify the presence of B trichothecenes and to evaluate the influence of each compound on different F. culmorum culture extracts. Preliminary results indicated that several molecules are able to inhibit the severity of F. culmorum in planta and its growth as well as trichothecene production in vitro. The level of inhibition of 3AcDON range from 67 to 100% under inducing conditions. Fast and effective methodologies for seed dressing were developed using a natural matrix.
- Subjects
TRICHOTHECENES; FUSARIUM oxysporum; BLIGHT diseases (Botany); FUSARIUM culmorum; MYCOTOXINS; HOST plants
- Publication
Journal of Plant Pathology, 2012, Vol 94, Issue 4, pS4.72
- ISSN
1125-4653
- Publication type
Article