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- Title
Canadian Association of Pathologists--Association canadienne des pathologistes National Standards Committee for High Complexity Testing/Immunohistochemistry.
- Authors
Cheung, Carol C.; Banerjee, Diponkar; Barnes, Penny J.; Berendt, Richard C.; Butany, Jagdish; Canil, Sarah; Clarke, Blaise A.; El-Zimaity, Hala; Garratt, John; Geldenhuys, Laurette; Gilks, C. Blake; Manning, Lisa; Mengel, Michael; Perez-Ordonez, Bayardo; Pilavdzic, Dragana; Riddell, Robert; Swanson, Paul E.; Torlakovic, Emina E.
- Abstract
Objectives: Formalin-fixed, paraffin-embedded unstained archived diagnostic tissue sections are frequently exchanged between clinical laboratories for immunohistochemical staining. The manner in which such sections are prepared represents a type of preanalytical variable that must be taken into account given the growing importance of immunohistochemical assays, especially predictive and prognostic tests, in personalized medicine. Methods: Recommendations were derived from review of the literature and expert consensus of the Canadian Association of Pathologists--Association canadienne des pathologists National Standards Committee for High Complexity Testing/Immunohistochemistry. Results: Relevant considerations include the type of glass slide on which to mount the unstained sections; the thickness of the tissue sections; the time from slide preparation to testing; the environment, particularly the temperature at which the unstained sections will be maintained prior to testing; the inclusion of on-slide positive control tissue where possible; and whether patient identifier(s) should be included on slide labels. Conclusions: Clear communication between requesting and releasing laboratories will facilitate the proper preparation of unstained sections and also ensure that applicable privacy considerations are addressed.
- Subjects
CANADA; PATHOLOGISTS; IMMUNOHISTOCHEMISTRY; HISTOCHEMISTRY; IMMUNOCHEMISTRY; SOCIETIES
- Publication
American Journal of Clinical Pathology, 2014, Vol 142, Issue 5, p629
- ISSN
0002-9173
- Publication type
Article
- DOI
10.1309/AJCP77GXYVEQXMXT