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- Title
Vibrational extraction QuEChERS for analysis of antiparasitic agents in fish by liquid chromatography coupled with tandem mass spectrometry.
- Authors
de Oliveira, Lenise Guimarães; Ramkumar, Abilasha; Moloney, Mary; Kurz, Márcia Helena Scherer; Gonçalves, Fábio Ferreira; Prestes, Osmar Damian; Danaher, Martin
- Abstract
A method was developed for the analysis of 22 antiparasitic residues belonging to the benzoylurea, organophosphate, pyrimidinamine, pyrethrin and pyrethroid classes in salmon by liquid chromatography coupled with tandem mass spectrometry. Samples were extracted with acetonitrile–water as the extraction solvent with use of a vibrational shaking apparatus with a ceramic homogenizer. After extraction, the acetonitrile extracts were cleaned up by incubation at low temperature (-20 °C, 1 h) to remove fat, followed by dispersive solid-phase extraction using Z-Sep+ and primary–secondary amine as sorbents. Validation was performed following the 2002/657/EC and SANTE/11813/2017 guidelines. The trueness of the method ranged from 87% to 121% and precision ranged from 4.1% to 23.7%, with the exception of cyphenothrin, dicyclanil and azamethiphos. The method developed is particularly advantageous because the use of a vibrational shaker allows unattended extraction of samples and eliminates a laborious tissue disruption step, which increases sample throughput in the laboratory. The sample preparation and chromatographic separations can be performed in 5 and 4 h, respectively, for 36 samples.
- Subjects
TANDEM mass spectrometry; ANTIPARASITIC agents; LIQUID chromatography; CHEMICAL sample preparation; LIQUID chromatography-mass spectrometry; SOLID phase extraction
- Publication
Analytical & Bioanalytical Chemistry, 2019, Vol 411, Issue 26, p6913
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-019-02062-z