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- Title
Direct Approach to Identification, at the Molecular Level, of Modified Proteins in Human Nuclear Cataractous Lenses: Beta-Crystallin is a Component of the Urea-Insoluble Protein Fraction.
- Authors
O'Hair, Richard A.J.; Sheil, Margaret M.; Truscott, Roger J.W.
- Abstract
In an effort to elucidate the molecular changes which take place in the human lens with the onset of nuclear cataract, the urea-insoluble protein fraction, solubilized with dithiothreitol, was digested with trypsin. Tryptic peptides separated by HPLC, were examined by both mass spectrometry and Edman degradation. A pentapeptide Gly-Glu-Tyr-Pro-Arg which is contained within the β-crystallin sequence was isolated. This finding provides direct evidence that β-crystallin is present in the urea-insoluble protein fraction which is known to be characteristic of human nuclear cataract lenses. Copyright © 1992 S. Karger AG, Basel
- Publication
Ophthalmic Research, 1992, Vol 24, Issue 5, p303
- ISSN
0030-3747
- Publication type
Article
- DOI
10.1159/000267183