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- Title
Transcriptional activation by Arabidopsis GT-1 may be through interaction with TFIIA–TBP–TATA complex.
- Authors
Le Gourrierec, José; Li, You-Fang; Zhou, Dao-Xiu
- Abstract
Summary GT-1 belongs to the class of trihelix DNA-binding proteins and binds to a promoter sequence found in many different genes. Data presented in this report show that GT-1 contains a trans-activation function in yeast and in plant cells. However, in tobacco BY-2 protoplasts, this activity functions only when an internal region containing the DNA-binding domain is deleted. Gel-shift and co-immunoprecipitation assays have revealed that GT-1 can interact with and stabilize the TFIIA–TBP–TATA complex. These results suggest that GT-1 may activate transcription through direct inter- action with the transcriptional pre-initiation complex.
- Subjects
GENETIC transcription; ARABIDOPSIS; PLANT genetics; GENETICS
- Publication
Plant Journal, 1999, Vol 18, Issue 6, p663
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1046/j.1365-313x.1999.00482.x