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- Title
HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system.
- Authors
Khamduang, Woottichai; Kaewbundit, Ampika; Duangmano, Amonrat; Hongjaisee, Sayamon; Klinbuayaem, Virat; Halue, Guttiga; Chutanunta, Apichat; Sirirungsi, Wasna; Jourdain, Gonzague; Ngo-Giang-Huong, Nicole
- Abstract
World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94) with a mean of differences of 0.23 log10 copies/mL. Using the 1,000 copies/mL cut-off, the sensitivity of DBS was 97% (95%CI, 91–100%) and specificity was 75% (95%CI, 19–99%). DBS are useful to scale-up HIV RNA VL testing in settings with limited access to VL testing.
- Subjects
THAILAND; WORLD Health Organization; RNA; VIRAL load; HIV; BLOOD; WORLD health; MEASUREMENT
- Publication
PLoS ONE, 2020, Vol 15, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0227929