We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The cation channel TRPM8 influences the differentiation and function of human monocytes.
- Authors
Hornsby, Eve; King, Hamish W.; Peiris, Madusha; Buccafusca, Roberto; Lee, Wing‐Yiu Jason; Wing, Elinor S.; Blackshaw, L. Ashley; Lindsay, James O.; Stagg, Andrew J.
- Abstract
Monocytes are mononuclear phagocytes that can differentiate to a variety of cell fates under the influence of their microenvironment and hardwired commitment. We found that inhibition of TRPM8 in human blood CD14+ monocytes during a critical 3‐h window at the beginning of their differentiation into macrophages led to enhanced survival and LPS‐driven TNFα production after 24 h. TRPM8 antagonism also promoted LPS‐driven TNFα production in CD14+ monocytes derived from the intestinal mucosa. Macrophages that had been derived for 6 days under blockade of TRPM8 had impaired phagocytic capacity and were transcriptionally distinct. Most of the affected genes were altered in a way that opposed normal monocyte to macrophage differentiation indicating that TRPM8 activity promotes aspects of this differentiation programme. Thus, we reveal a novel role for TRPM8 in regulating human CD14+ monocyte fate and function.
- Subjects
MONOCYTES; MACROPHAGES; CD14 antigen; INTESTINAL mucosa; TRP channels
- Publication
Journal of Leukocyte Biology, 2022, Vol 112, Issue 3, p365
- ISSN
0741-5400
- Publication type
Article
- DOI
10.1002/JLB.1HI0421-181R