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- Title
MICROSATELLITE MARKER-BASED MOLECULAR CHARACTERIZATION OF SMALL AND MEDIUM-GRAINED AROMATIC RICE GERMPLASM OF ODISHA, INDIA.
- Authors
VENKATESAN, K.; BHAT, K. V.
- Abstract
Aromatic rice is a kind of specialty rice of the world and is nature's gift to Indo-Pak region. The study aims to evaluate the genetic diversity and relationship among 40 aromatic rice comprising 34 small and medium-grained aromatic rice along with 6 long-grained Basmati rice through microsatellite marker (SSR) analysis using 24 primerpairs, of which 22 (91.6%) were polymorphic. In total, 51 alleles were detected for 22 polymorphic primer-pairs, with an average of 2.3 alleles per locus. Polymorphism Information Content (PIC) values ranged from 0.05 to 0.57 with an average of 0.33. Four SSR loci revealed PIC values higher than 0.50 and are RM224, RM257, RM241 and RM217. The genotype 'Ganjeikalli' discriminated from rest of genotypes and the basmati-types distinguished from non-basmati types by SSR markers, 'RM249' and 'RM104', respectively. Dendrogram based on the cluster analysis by microsatellite polymorphism, grouped 40 aromatic rice genotypes into 2 major clusters at simple matching (SM) coefficient value of 0.48. Both cluster I and II were divided into 2 subclusters at SM coefficient value of 0.66 and 0.76 respectively, and are further formed sub-subclusters at below subcluster level on basis of eco-geographic regions of Odisha. Clustering pattern obtained in the study revealed that individuals present in cluster-I was more diverse than cluster-II. In subcluster II (i), genotype-pairs Jhingisiali and DP-24, 3114-1 and IC-257505, IC-283204 and IC-283311 were showed extreme similarity (100%) between them. Principal Coordinate Analysis (PCoA) explained that the amount of genetic variation present in SSR molecular data was 63.4% through its cumulative value of first 3 coordinates. Based on this study, the use of SSR marker as a tool to evaluate genetic diversity and their relatedness, which is essential for varietal identification, classification, purity maintenance and conservation of indigenous scented rice germplasm, was elucidated.
- Subjects
ODISHA (India); RICE genetics; MICROSATELLITE repeats in plants; RICE quality; PLANT germplasm
- Publication
SABRAO Journal of Breeding & Genetics, 2015, Vol 47, Issue 3, p248
- ISSN
1029-7073
- Publication type
Article