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- Title
Dermokine: An Extensively Differentially Spliced Gene Expressed in Epithelial Cells.
- Authors
Naso, Michael F.; Liang, Bailin; Huang, C. Chris; Xiao-Yu Song; Shahied-Arruda, Lillian; Belkowski, Stanley M.; D'Andrea, Michael R.; Polkovitch, Debbie A.; Lawrence, Danielle R.; Griswold, Don E.; Sweet, Ray W.; Amegadzie, Bernard Y.
- Abstract
Studies performed to discover genes overexpressed in inflammatory diseases identified dermokine as being upregulated in such disease conditions. Dermokine is a gene that was first observed as expressed in the differentiated layers of skin. Its two major isoforms, α and β, are transcribed from different promoters of the same locus, with the α isoform representing the C terminus of the β isoform. Recently, additional transcript variants have been identified. Extensive in silico analysis and reverse transcriptase (RT)-PCR cloning has confirmed the existence of these variants in human cells and tissues, identified a new human isoform as well as the γ isoform in mouse. Recombinant expression and analysis of the C-terminal truncated isoform indicate that the molecule is O-linked glycosylated and forms multimers in solution. In situ hybridization and immunohistochemistry has shown that the gene is differentially expressed in various cells and tissues, other than the skin. These results show that the dermokine gene is expressed in epithelial tissues other than the skin and this expression is transciptionally and posttranscriptionally complex.Journal of Investigative Dermatology (2007) 127, 1622–1631; doi:10.1038/sj.jid.5700779; published online 22 March 2007
- Subjects
EPITHELIAL cells; REVERSE transcriptase; DNA polymerases; IN situ hybridization; IMMUNOHISTOCHEMISTRY; SKIN diseases
- Publication
Journal of Investigative Dermatology, 2007, Vol 127, Issue 7, p1622
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/sj.jid.5700779