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- Title
Effect of airway delivered type II alveolar epithelial cells on mice with bleomycin-induced lung injury.
- Authors
Ping Ming Wang; Martin II, William J.
- Abstract
Bleomycin is known to injure the alveolar epithelium in vivo and cause pulmonary fibrosis. Type II alveolar epithelial (AT II) cells are progenitor cells of the adult alveolar epithelium. We hypothesize that the airway delivery of donor AT II cells will improve survival of C57BL/6 mice treated with IT bleomycin. To address this, female mice (10-12 weeks) were given either bleomycin (0.06 U in 40 µl saline solution) or saline (40 µl) intratracheally. We harvested and purified AT 11 cells from normal male murine lungs, and we airway delivered the donor AT II cells (1-2 x 10^6) in 40 µl medium at day 2 or day 5 post-bleomycin treatments. After 3-weeks following bleomycin treatment, the survival rate of bleomycin-treated control mice with vehicle alone at day 2 or 5 post-bleomycin was 13 ± 18% or 27 ± 4.0% (mean ± SD, total of 17 mice). The survival rate of mice treated with donor ATII cells at day 2 post-bleomycin was 17 ± 23% (total of 18 mice). In contrast, bleomycin-injured mice treated with airway delivered ATII cells at day 5 post-bleomycin resulted in a survival of 78 ± 31% (total of 11 mice). This finding was further supported by improvement in weight loss and lung compliance in the mice that received the ATII cell treatment. Our preliminary study suggests that donor AT II cells may act as effective repair cells for acute lung injury.
- Subjects
AIRWAY (Anatomy); EPITHELIAL cells; PULMONARY alveoli; BLEOMYCIN; LUNG injuries; PULMONARY fibrosis; PHYSIOLOGIC salines; LABORATORY mice
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA555
- ISSN
0892-6638
- Publication type
Article