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- Title
Decreased oxidative DNA damage in Ogg1/Myh-deficient mice treated with pomegranate extract.
- Authors
Henning, Susanne M.; Reliene, Ramune; Schiestl, Robert H.; Yamamoto, Mitsuko Lynn; Luyi Li; Seeram, Navindra; Heber, David
- Abstract
Oxidative DNA damage is linked to carcinogenesis. Base excision repair (BER) is critical for the repair of oxidative DNA lesions such as 8-hydroxy-2′-deoxyguanosine (8OHdG). 8-oxoguanine-DNA glycosylase 1 (hOGG1) activity comprises the first step of the BER pathway. The mutY gcne encodes an additional DNA glycosylase (Myh), which removes adenosine when paired with 8OhdG to accommodate the removal of 8OHdG. The ratio of 8OhdG to total dG as determined by high performance liquid chromatography (HPLC) was increased in the DNA extracted from tissues of mice deficient in Ogg /Myh. After daily treatments with 0.8 mg of PomX for 23 days the ratio 8OhdG/dG was decreased by 30, 55 and 33% in liver, kidney and lung, respectively, compared to vehicle control treated mice. Total strand breaks in lymphocytes from whole blood of the same mice as determined by comet assay were decreased by 49% as well. Pomegranate extract (PomX) was prepared from skins and arils minus the seeds and contains 37% of punicalagin (hydrolysable ellagitannins), which is hydrolysed in the intestine to release ellagic acid (EA). EA in turn can be metabolized to urolithins in the intestine and absorbed into the circulation. Using LC/ESI-MS analysis trace amounts of EA metabolites were found in the tissues of mice treated with PomX. We conclude that pomegranate phytochemicals protect against oxidative DNA damage through their strong antioxidant activity.
- Subjects
DNA damage; CARCINOGENESIS; LABORATORY mice; ADENOSINES; DNA; LYMPHOCYTES
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA366
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.5.a366-b