We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Roscovitine inhibits ERK1/2 activation induced by angiotensin II in vascular smooth muscle cells
- Authors
Li, Ai-Ying; Han, Mei; Zheng, Bin; Wen, Jin-Kun
- Abstract
Abstract: Roscovitine is a potent CDK inhibitor often used as a biological tool in cell-cycle studies, but its working mechanism and real targets in vascular smooth muscle cells (VSMCs) remain unclear. In this study, we observed that ERK1/2 phosphorylation induced by Ang II was abrogated by pretreating VSMCs with roscovitine for 15h. Pretreating VSMCs with roscovitine also inhibited Ang II-induced c-Jun expression and phosphorylation. We further demonstrated that roscovitine could suppress the DNA binding activity of c-Jun and activation of angiotensinogen promoter by Ang II. These results suggest that roscovitine represses Ang II-induced angiotensinogen expression by inhibiting activation of ERK1/2 and c-Jun.
- Subjects
SMOOTH muscle; VASCULAR smooth muscle; BLOOD vessels; MUSCLE cells
- Publication
FEBS Letters, 2008, Vol 582, Issue 2, p243
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.12.011