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- Title
The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization
- Authors
Geng, Hua; Liu, Yuet-Man; Chan, Wai-Sing; Lo, Anthony Wing-Ip; Au, Deborah Mun-Yee; Waye, Mary Miu-Yee; Ho, Yuan-Yuan
- Abstract
Abstract: The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.
- Subjects
ORGANS (Anatomy); PRESERVATION of organs, tissues, etc.; IMMUNOHISTOCHEMISTRY; CONFOCAL microscopy
- Publication
FEBS Letters, 2005, Vol 579, Issue 30, p6763
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.11.007