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- Title
Spermine is not essential for survival of Arabidopsis
- Authors
Imai, Akihiro; Akiyama, Takashi; Kato, Tomohiko; Sato, Shusei; Tabata, Satoshi; Yamamoto, Kotaro T.; Takahashi, Taku
- Abstract
Spermine is the final product of the polyamine biosynthetic pathway and is ubiquitously present in most organisms. The genome of Arabidopsis thaliana has two genes encoding spermine synthase: ACAULIS5 (ACL5), whose loss-of-function mutants show a severe defect in stem elongation, and SPMS. In order to elucidate the function of spermine in plants, we isolated a T-DNA insertion mutant of the SPMS gene. Free and conjugated spermine levels in the mutant, designated spms-1, were significantly decreased compared with those in the wild-type, but no obvious morphological phenotype was observed in spms-1 plants. We further confirmed that acl5-1 spms-1 double mutants contained no spermine. Surprisingly, acl5-1 spms-1 was fully as viable as the wild-type and showed no phenotype except for the reduced stem growth due to acl5-1. These results indicate that spermine is not essential for survival of Arabidopsis, at least under normal growth conditions.
- Subjects
SPERMINE; ARABIDOPSIS thaliana; PHENOTYPES; ENZYMES
- Publication
FEBS Letters, 2004, Vol 556, Issue 1-3, p148
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01395-4