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- Title
Adeno-associated virus serotype 2 mediated transduction and coexpression of the human apoAI and SR-BI gene in HepG2 cells.
- Authors
Li, Bingnan; Zhang, Juan; Li, Zhiyan; Tan, Mengqun
- Abstract
Cholesterol efflux is the first step in the reverse cholesterol transport (RCT) pathway, removing excess cholesterol from tissues, including the arterial wall, thus preventing the development of atherosclerosis. Adeno-associated virus (rAAV) has demonstrated significant promise as a DNA-delivery vector to treat serious human diseases. In this study, we constructed recombinant adeno-associated viruses coexpressing apoAI and SR-BI successfully, the double gene mRNA and protein were both strongly expressed in transduced HepG2 cells. A novel safe and efficient method of promoting the reverse cholesterol transport (RCT) may be established. These results may provide a new method for gene therapy of Arteriosclerosis.
- Subjects
GENETIC transformation; APOLIPOPROTEIN A; ATHEROSCLEROSIS; GENE therapy; ADENO-associated virus
- Publication
Molecular Biology Reports, 2012, Vol 39, Issue 1, p25
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-011-0706-5