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- Title
Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development.
- Authors
Oommen, Shelly; Otsuka-Tanaka, Yoko; Imam, Najam; Kawasaki, Maiko; Kawasaki, Katsushige; Jalani-Ghazani, Farnoosh; Anderegg, Angela; Awatramani, Rajeshwar; Hindges, Robert; Sharpe, Paul T.; Ohazama, Atsushi
- Abstract
Background: Tooth development is known to be mediated by the cross-talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19- to 25-nt noncoding small single-stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be important for the fine-tuning signaling pathways in development. To investigate the role of miRNAs in tooth development, we examined mice with either mesenchymal ( Wnt1Cre/Dicer fl/fl) or epithelial ( ShhCre/Dicer fl/fl) conditional deletion of Dicer, which is essential for miRNA processing. Results: By using a CD1 genetic background for Wnt1Cre/Dicer fl/fl, we were able to examine tooth development, because the mutants retained mandible and maxilla primordia. Wnt1Cre/Dicer fl/fl mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Extra incisor tooth formation was found in ShhCre/Dicerfl/fl mice, whereas molars showed no significant anomalies. Microarray and in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars. Conclusion: In tooth development, miRNAs thus play different roles in epithelium and mesenchyme, and in incisors and molars. Developmental Dynamics 241:1465-1472, 2012. © 2012 Wiley Periodicals, Inc.
- Publication
Developmental Dynamics, 2012, Vol 241, Issue 9, p1465
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.23828